spark wellplate reader (Tecan Systems)
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99
Structured Review
Tecan Systems
spark wellplate reader
Spark Wellplate Reader, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 99/100, based on 17046 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wellplate+reader/Spark/pm40883160-75-29-33
Average 99 stars, based on 17046 article reviews
Spark Wellplate Reader, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 99/100, based on 17046 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wellplate+reader/Spark/pm40883160-75-29-33
Average 99 stars, based on 17046 article reviews
spark wellplate reader - by Bioz Stars,
2026-09
99/100 stars
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Binding Assay:Article Title: Designed Ankyrin Repeat Proteins as Actin Labels of Distinct Cytoskeletal Structures in Living Cells. Article Snippet: For this purpose, crude extracts of DARPin clones or purified DARPins diluted (1:1000 (screens 1 and 2) or 100 nM (quantitative ELISA)) in PBS-TB (PBS pH 7.4, 0.1% (v/v) Tween-20, 0.2% (w/v) bovine serum albumin (BSA) (supplemented with 1 mM ATP, 5 mM DTT and 50 mM MgCl2 (for ELISA with F-actin)/2 mM CaCl2 (for ELISA with Gactin) for actin stability) were incubated with 50 nM biotinylated Factin (screen 1) or 50 nM G-actin (screen 2 and quantitative ELISA) immobilized on precoated neutravidin (screen 1 and 2) or streptavidin (quantitative ELISA) for 1 h at room temperature. https://doi.org/10.1021/acsnano.3c12265 ACS Nano 2024, 18, 8919−8933 8928 Subsequently, target-specific binding of DARPins was detected after each 1 h incubation at room temperature with a mouse α-FLAG RGS(His)4 IgG1 (clone M2) (Sigma-Aldrich; F3165) (1:5000 in PBS-TB) and an alkaline phosphatase -conjugated polyclonal goat αmouse-IgG antibody as secondary antibody (Sigma-Aldrich; A3562) (1:10000 in PBS-TB). .. Target-specific binding of DARPins was analyzed by following the color change of the hydrolysis of 3 mM |